Specifications

Below are standard specifications for plastic PCR tubes. Items marked Customizable are available upon request.

Size	Material	Capacity (µL)	Cap Type	Color	Wall	Sterile
0.2ml Flat	PP	200	Flat	Clear/Color	Ultra-thin	Gamma
0.2ml Domed	PP	200	Domed	Clear/Color	Ultra-thin	Gamma
0.5ml Flat	PP	500	Flat	Clear/Color	Ultra-thin	Gamma
0.5ml Domed	PP	500	Domed	Clear/Color	Ultra-thin	Gamma

Color options: red, blue, yellow, green, orange, purple, etc. 0.5ml size compatible with large-volume PCR instruments.

📌 Customization Notes:
• Custom sizes available (0.2ml / 0.5ml)
• Cap type options: flat / domed / 8-strip caps
• Color options: clear / color
• 8-strip tube format available
• Low-binding treatment available

Product Description

I. Overview

The plastic PCR tube is an essential consumable for sample loading in polymerase chain reaction (PCR) experiments. Manufactured from high-purity polypropylene (PP) through precision injection molding, featuring uniform wall thickness and smooth inner surfaces for efficient PCR reactions.

Available in 0.2ml and 0.5ml sizes, compatible with most major PCR instruments. Caps available in flat and domed styles — flat caps are suitable for quantitative PCR (qPCR), domed caps for conventional PCR. Available in clear and color (red, blue, yellow, green, etc.) options for easy sample identification.

Ultra-thin wall design ensures efficient heat transfer, tight cap sealing prevents reaction system evaporation and cross-contamination. Sterilized by gamma irradiation, DNase/RNase-free, pyrogen-free, ensuring accurate and reliable experimental results.

🔬 Product Advantages:
High-Purity PP — Low adsorption, ensures reaction system stability
Ultra-Thin Wall — Efficient heat transfer, reduces cycling time
Excellent Sealing — Prevents reaction system evaporation
Multiple Colors — Color tubes enable easy sample identification
DNase/RNase-free — Pyrogen-free, ensures experimental accuracy

II. Core Performance Parameters

Material: High-purity polypropylene (PP)
Process: Precision injection molding
Sizes: 0.2ml / 0.5ml
Cap Types: Flat / Domed
Colors: Clear / Color (red/blue/yellow/green/orange/purple etc.)
Wall Thickness: Ultra-thin (approx. 0.15mm)
Sterilization: Gamma irradiated
Quality: DNase/RNase-free, pyrogen-free
Temperature Range: -20℃ ~ 121℃
Compatibility: major PCR instruments

III. Material & Workmanship

Manufactured from medical-grade polypropylene (PP) resin in a cleanroom environment. Tube walls are uniformly thin with smooth inner surfaces to minimize sample adsorption. Ultra-thin wall design ensures efficient heat transfer, reducing PCR cycling time.

Caps are manufactured with precision molds to ensure tight sealing, preventing reaction system evaporation during high-temperature cycling. Color tubes use medical-grade masterbatch that is free from harmful substances and does not affect PCR reactions.

Every batch undergoes rigorous dimensional, visual, and enzyme-free testing to ensure consistent quality.

Applications

Typical Applications

Gene Amplification

Conventional PCR, nested PCR, long-fragment PCR

Quantitative PCR

SYBR Green method, TaqMan probe qPCR

RT-PCR

Reverse transcription PCR, real-time RT-PCR

Genotyping

SNP detection, gene mutation detection

Molecular Cloning

Target gene fragment amplification

Pathogen Detection

Microbial and viral nucleic acid detection

Industries

Teaching Labs Molecular biology education
Pharmaceutical Drug discovery, QC labs
Clinical Diagnostics Clinical testing, pathogen detection
Biotechnology Genetic engineering, biopharmaceuticals
Research Institutions Molecular biology and genetics laboratories

Selection Guide

I. By Cap Type

Experiment Type	Recommended Cap	Reason
Quantitative PCR (qPCR)	Flat Cap	Flat caps are ideal for fluorescence signal collection with good light transmission
Conventional PCR, RT-PCR	Domed / Flat	Both options are suitable
Multi-channel fluorescence detection	Flat Cap	Minimizes optical interference

II. By Color

Application	Recommended Color	Use Case
Routine experiments, fluorescence detection	Clear	Compatible with most PCR instruments, good fluorescence transmission
Multiple sample differentiation	Color	Different colors for different samples or experimental groups
Light-sensitive reactions	Color (dark)	Reduces light interference with reactions

III. By Capacity

0.2ml: Routine PCR, qPCR (most common)
0.5ml: Large-volume PCR, long-fragment amplification

💡 Selection Tip: 0.2ml flat cap clear PCR tube is the most commonly used size, compatible with most qPCR instruments.
For multi-sample management, choose color PCR tubes.
For large-volume amplification, choose 0.5ml size.
For high-throughput operations, choose 8-strip tube format.

Operation & Maintenance

User Guide

Below are standard operating guidelines for plastic PCR tubes. Proper use ensures PCR accuracy and reliability.

I. Pre-use Preparation

Inspect packaging integrity, confirm no damage
Check the sterilization expiry date
Work in a laminar flow hood or PCR-dedicated area
Prepare PCR reaction system (primers, template, enzyme, buffer, etc.)

II. PCR Operation

Remove PCR tube from packaging in the laminar flow hood
Add the prepared PCR reaction system to the tube
Do not exceed the marked capacity
Close the cap securely (press until you hear a "click")
Label the cap or tube body with sample ID
Place the PCR tube in the PCR instrument and start the program

III. Disposal

Dispose of used PCR tubes in biohazard waste containers
PCR tubes containing nucleic acid samples should be disposed according to biosafety regulations

💡 From a Seasoned Lab Technician: "Seal the cap firmly to prevent evaporation" — In PCR reactions, improperly sealed caps cause reaction system evaporation, affecting results.
"Flat for qPCR, domed for routine PCR" — qPCR requires flat caps to minimize optical interference.
"Color tubes for easy identification" — Use different color PCR tubes to distinguish experimental groups or samples.
"8-strip tubes save time" — For high-throughput experiments, use 8-strip tubes to increase loading efficiency.

Care & Notes

Show care & notes

⚠️ Safety & Compliance Checklist

Personal Protection

Wear lab coat and gloves during operation

Biosafety

Follow appropriate biosafety guidelines when handling biohazardous samples

Do not

Aseptic Technique: Work in a laminar flow hood or PCR-dedicated area to avoid contamination
Avoid Cross-Contamination: Use different PCR tubes for different samples

Plastic PCR Tube

Specifications

Product Description

I. Overview

II. Core Performance Parameters

III. Material & Workmanship

Applications

Gene Amplification

Quantitative PCR

RT-PCR

Genotyping

Molecular Cloning

Pathogen Detection

Selection Guide

I. By Cap Type

II. By Color

III. By Capacity

User Guide

I. Pre-use Preparation

II. PCR Operation

III. Disposal

Care & Notes

Personal Protection

Biosafety

Need Plastic PCR Tubes?

Plastic PCR Tube

Specifications

Product Description

I. Overview

II. Core Performance Parameters

III. Material & Workmanship

Applications

Gene Amplification

Quantitative PCR

RT-PCR

Genotyping

Molecular Cloning

Pathogen Detection

Selection Guide

I. By Cap Type

II. By Color

III. By Capacity

User Guide

I. Pre-use Preparation

II. PCR Operation

III. Disposal

Care & Notes

Personal Protection

Biosafety

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