Specifications

Chromatography Column · Specifications

Standard sizes are listed below. All products comply with GB/T, ASTM, and ISO standard taper systems. Full range 10/19 ~ 60/45 available. Custom IDs, effective lengths, and wall thicknesses are available.

Request custom specs

1. Standard Chromatography Column Sizes

Note: Length notation varies by brand. Some professional brands specify "effective length" (from frit top to tube mouth, excluding the lower outlet tube). Some domestic brands specify "total length" (including the joint and lower outlet). Please check the actual product for precise dimensions.

ID (mm)	OD (mm) approx.	Effective Length (mm)	Common Joint	Wall Thickness (mm)	Separation Scale
10 ~ 13.4	13 ~ 17	200 / 300 / 500 / 1000	14# / 19# / 24#	1.5 ~ 2.0	Micro (< 50mg)
16 ~ 20	20 ~ 26	200 / 300 / 500 / 1000	19# / 24#	2.0 ~ 3.0	Small (50-500mg)
26 ~ 32	32 ~ 40	200 / 300 / 500 / 1000	24# / 29#	2.5 ~ 3.0	Medium (500mg-5g)
40 ~ 46	46 ~ 53	200 / 300 / 500 / 1000	19# / 22# / 24#	3.0	Preparative
53 ~ 60	60 ~ 67	200 / 300 / 500 / 1000	24# / 29#	3.5	Large-scale
73 ~ 80	80 ~ 87	300 / 500 / 1000	24# / 29#	3.5	Batch (> 5g)
90 ~ 100	100 ~ 110	300 / 500 / 700 / 800 / 900 / 1000	24# / 29# / 34#	4.0+	Industrial
100+	110+	1000 / 1500 / 2000 / 2500	24# / 29# / 34# / 40#	4.0+	Mass Production

2. Standard Joint Specifications (GB/ASTM/ISO)

GB/T	ASTM	ISO
10#	10/19	10/18
14#	14/20	14/23
19#	19/22	19/26
24#	24/40	24/29
29#	29/42	29/32
34#	34/45	34/35
40#	40/50	40/38

3. Length Selection Guide

Effective Length	Typical Application
200 ~ 300mm	Short column, rapid separation, simple mixtures, teaching labs
400 ~ 500mm	Standard length, most routine laboratory separations
600 ~ 800mm	Extended length, improved resolution, complex mixtures
900 ~ 1000mm	Long column, high resolution, preparative purification
1000 ~ 1500mm	Extra-long column, industrial preparative, large-scale purification
1500 ~ 2500mm	Industrial ultra-long column, mass production, high‑standard separation
2500mm+	Custom special orders, specific industrial applications

* Joint numbers (14#, 19#) indicate the largest diameter (mm) of the taper. All are 1:10 taper.
* GB/ASTM/ISO designations are different standard identifiers — physical dimensions are identical and fully interchangeable.
* Full range 10/19 ~ 60/45 available upon request.
* IDs from 10mm to 220mm+ available upon request.
* Effective lengths from 200mm to 2500mm+ available upon request.
* Contact our sales team for MOQ and lead time on custom orders.

✦ Customization Services — We offer custom IDs, effective lengths, joint sizes, and wall thicknesses. Share your specific requirements and our technical team will provide a tailored solution.

Product Description

The chromatography column is the essential glassware for column chromatography separation and purification. Its straight glass tube body features a standard taper joint at the upper end for connection to solvent reservoirs, constant-pressure funnels, or chromatography accessories, and an outlet at the lower end for fraction collection.

Manufactured from high-borosilicate glass 3.3, the column offers excellent clarity for real-time observation of bed height, sample migration bands, and separation performance. Precision inner diameter control ensures consistent column efficiency, while the standard taper joint design enables flexible combination with a wide range of standard joint accessories for different chromatography modes (normal phase, reverse phase, gel filtration, ion exchange, etc.).

Available in inner diameters from 10mm to 100mm+ and effective lengths from 200mm to 2500mm and beyond, covering applications from micro-analysis and laboratory-scale separations to industrial-scale preparative chromatography. Custom lengths (1000mm, 1500mm, 2000mm, and more) are available upon request.

Key Features

Borosilicate Glass 3.3: Meets ISO 3585, low thermal expansion, thermal shock resistance up to 280°C, excellent chemical durability
Multi‑Standard Compliance: Precision‑ground 1:10 taper meets GB/T, ASTM E, and ISO 3585 standard taper specifications for full interchangeability
High Clarity: >92% light transmission for real-time bed observation
Precision ID: Accurate inner diameter control ensures consistent column efficiency and separation reproducibility
Extended Length Options: Effective lengths up to 2500mm and beyond for industrial-scale preparative applications
Thick-Wall Design: Enhanced strength and safety using premium borosilicate glass

Quality Assurance

Each chromatography column is inspected for dimensional accuracy, taper precision, and surface finish to ensure reliable sealing with all standard joint components. Custom bulk orders are welcome.

Tip: Ensure joints are clean and dry before use. Use a column frit or glass wool at the bottom to prevent packing loss. Extra-long columns (>1000mm) require special attention to vertical mounting and support to prevent bending or breakage. Handle glass columns with care.

Applications

Typical Uses

Column Chromatography

Purification of natural products, synthetic compounds, organic intermediates

Protein Purification

Affinity, ion exchange, and gel filtration chromatography

Environmental Analysis

Enrichment and separation of organic pollutants in water samples

Pharmaceutical R&D

Purification of drug intermediates, impurity separation

Industrial Production

Large-scale preparation, bulk purification (extra-long columns)

Teaching Labs

Chromatography education at universities and research institutes

Compatible Equipment

Standard joint solvent reservoirs, constant-pressure funnels

Chromatography accessories (stopcocks, frits, adapters)

Ring stands and clamps (specialized supports for extra-long columns)

Peristaltic pumps, fraction collectors

Tip: Always use matching standard joint sizes for optimal sealing. Columns can be flexibly combined with other standard joint glassware to build complete chromatography systems. Extra-long columns (>1000mm) should be used with specialized support stands for safety.

Operation & Maintenance

User Guide

Before Use

Inspect for cracks, chips, or scratches on any joint
Clean all joint surfaces with a soft cloth to remove particulates
Confirm that the joint size matches your accessories
Prepare packing material, eluent, and collection vessels
For extra-long columns, confirm that the support stand is secure

Packing Procedure

Place a small amount of glass wool or a frit at the bottom as support
Mount the column vertically on a stand or specialized support
Add a small volume of eluent to wet the column
Slowly add packing material while gently tapping the column for even settling
Add a layer of glass wool or filter paper on top of the packing

Chromatography Operation

Connect the upper joint to a solvent reservoir or constant-pressure funnel
Apply the sample and begin elution
Collect fractions based on the separation profile
Adjust flow rate to optimize separation

Cleaning & Storage

Rinse immediately after use with appropriate solvent
Use soft brushes and appropriate detergents for the tube interior
Air dry and store with protective paper between ground joints
Protect joints from impacts
Extra-long columns: store horizontally on dedicated racks to prevent bending