Name: Conical Centrifuge Tube
Brand: RichTech

Specifications

Conical Centrifuge Tube · Specifications

The following are standard capacity options. Custom capacities or special graduation markings are available upon request.

Request custom specs

Capacity (mL)	Approx. Diameter (mm)	Approx. Height (mm)	Bottom Type	Graduations	Material
5	12	55	Conical	Yes	Borosilicate 3.3
10	14	75	Conical	Yes	Borosilicate 3.3
15	16	95	Conical	Yes	Borosilicate 3.3
25	20	115	Conical	Yes	Borosilicate 3.3
30	22	125	Conical	Yes	Borosilicate 3.3
50	28	130	Conical	Yes	Borosilicate 3.3

* Diameter and height are approximate values. Actual dimensions may vary slightly.
* Custom capacities and special graduations available upon request. Bulk pricing available.
* Optional rubber cushions or silicone adapters are available to reduce wall abrasion during centrifugation.

Rotor Compatibility: These tubes are designed for standard centrifuge rotors with tube apertures of 13mm–30mm. For high-speed or large-volume applications, we strongly recommend using rubber cushions or adapters to distribute stress and extend tube service life.

Product Description

Conical centrifuge tubes are essential for laboratory centrifugation and sample preparation. Manufactured from high-quality borosilicate glass 3.3, the tapered conical design ensures that pelleted materials are tightly packed at the tube tip, facilitating efficient supernatant removal and pellet recovery. Uniform wall thickness and high mechanical strength allow these tubes to withstand standard centrifugation forces (up to 5,000 × g) across a wide range of rotor types.

Key Features

Borosilicate Glass 3.3: ISO 3585 compliant · Low thermal expansion · Thermal shock resistance up to 280°C · Resistant to acids, alkalis, and organic solvents
Conical Bottom Design: Pellet concentration at the tube tip for easy observation, transfer, and resuspension
High Mechanical Strength: Uniform wall construction ensures reliable performance under standard RCF conditions (up to 5,000 × g)
Superior Clarity: High-purity glass enables clear visualization of pellet formation and color changes
Clear Graduations: Permanent volume markings for quantitative sample handling
Autoclavable: Steam sterilization at 121°C / 15 psi for 20 minutes
Chemical Resistant: Compatible with acids, alkalis, salt solutions, and common organic solvents

Quality Assurance

Each centrifuge tube undergoes rigorous dimensional inspection, wall thickness uniformity testing, and mechanical strength verification to ensure safe and reliable performance under high-speed centrifugation. Our products meet laboratory consumable standards, delivering consistent, reproducible results for your sample preparation workflows.

Note: Glass centrifuge tubes should be inspected for cracks or chips before each use. Always use appropriate tube adapters or rubber cushions to avoid direct hard contact with rotors. Select cleaning and sterilization methods appropriate for your specific sample types.

Applications

Typical Application Areas

Biochemistry & Molecular Biology

Cell lysate clarification, protein precipitation, nucleic acid extraction (solid-liquid separation)

Clinical Diagnostics

Blood sample centrifugation (serum/plasma separation), urine sediment analysis, CSF cell collection

Microbiology

Bacterial culture pellet collection, cell washing, spore separation

Pharmaceutical QC

Impurity separation in drug raw material testing, sedimentation in formulation stability studies

Environmental & Food Testing

Suspended solids collection from water samples, solid-liquid separation in food sample preparation

Compatible Equipment

All major benchtop centrifuge brands (major brands)

Floor-model large-capacity centrifuge standard racks/rotors

Microcentrifuges (with 1.5ml / 2ml adapter inserts)

Refrigerated centrifuges (equally suitable for low-temperature operation)

Tip: Conical centrifuge tubes work together with conical graduated tubes and round-bottom graduated tubes to meet diverse experimental needs. For best results, pair with RichTech pipettes, graduated cylinders, and other volumetric products for a complete sample preparation workflow.

Conical Centrifuge Tube Operation Guide

03

Preparation Before Use

Inspect each tube for cracks, chips, or rim damage — damaged tubes must never be used for centrifugation
Ensure tube walls are clean and transparent — free from residues or grease
Rinse 2–3 times with distilled water and dry before use (or sterilize as required by protocol)
Autoclave conditions: 121°C / 15 psi / 20 minutes

04

Centrifugation Procedure

Counterbalance precisely: Tubes must be symmetrically placed with weight deviation ≤ 0.1g to protect the rotor bearing
Fill volume: Do not exceed 80% of the nominal capacity to prevent spillover during centrifugation
Rotor matching: Ensure tubes fit the rotor aperture — use rubber cushions or adapters when necessary
Centrifuge settings: Adjust speed and time according to sample type. Recommended RCF for glass tubes: ≤ 5,000 × g
Acceleration/Braking: Use medium acceleration/deceleration settings to avoid sudden stress on the glass

05

Post-Centrifugation Handling

Allow tubes to stand for 2–3 minutes before opening to prevent aerosol dispersion
Use a pipette or transfer pipette to carefully aspirate the supernatant without disturbing the pellet
For pellet collection, resuspend in a small volume of liquid and transfer as needed

06

Cleaning & Maintenance

Clean immediately after use to prevent sample residues from adhering
Routine cleaning: Mild detergent + soft brush — rinse 3 times with distilled water
Stubborn residues: Soak in dilute nitric acid or specialized cleaning solution for 2–4 hours
Invert to air dry or place in oven at 60°C until dry
Store upright in dedicated tube racks to avoid impact and crushing

Care & Notes

Show care & notes

Safety Precautions

Always secure caps or seal with Parafilm during centrifugation
When working with biohazardous, corrosive, or highly toxic samples, perform all steps in a biosafety cabinet or fume hood
Glass centrifuge tubes are not suitable for dry-heat sterilization (above 160°C) — use autoclaving instead
If unusual vibration or noise occurs during centrifugation, stop immediately and inspect

Do not

Never use cracked or damaged centrifuge tubes — they may shatter at high speed
Open tubes slowly and gently after centrifugation to avoid aerosol generation