Name: Conical Graduated Centrifuge Tube
Brand: RichTech

Specifications

Conical Graduated Centrifuge Tube · Specifications

The following are standard capacities and graduation ranges. Custom capacities or special graduation intervals are available upon request.

Request custom specs

Nominal Capacity (mL)	Graduation Range (mL)	Graduation Interval (mL)	Approx. Diameter (mm)	Approx. Height (mm)	Bottom Type	Material
5	0.5 – 5	0.5	12	55	Conical	Borosilicate 3.3
10	1 – 10	1.0	14	75	Conical	Borosilicate 3.3
15	1 – 15	1.0	16	95	Conical	Borosilicate 3.3
25	2 – 25	1.0	20	115	Conical	Borosilicate 3.3
30	2 – 30	1.0	22	125	Conical	Borosilicate 3.3
50	5 – 50	2.0	28	130	Conical	Borosilicate 3.3

* Diameter and height are approximate values. Actual dimensions may vary slightly.
* Custom graduation intervals available (e.g., 0.2ml, 0.5ml, 1.0ml, 2.0ml).
* Non-graduated versions (standard conical tubes) and custom markings also available.

Marking Technology: Graduations are applied using high-temperature fused white enamel that bonds with the glass surface, providing exceptional resistance to acids, alkalis, organic solvents, and repeated cleaning cycles.

Product Description

Conical graduated centrifuge tubes are a versatile upgrade to standard conical tubes, incorporating precise volume graduations directly on the tube body. Manufactured from high-quality borosilicate glass 3.3, the tapered conical design concentrates pelleted materials at the tube tip, while the clear, permanent graduations enable direct volume measurement before and after centrifugation. This integrated design eliminates the need for sample transfer to separate graduated cylinders or test tubes, reducing sample loss and measurement errors — particularly valuable for precious samples and micro-volume applications.

Key Features

Borosilicate Glass 3.3: ISO 3585 compliant · Low thermal expansion · Thermal shock resistance up to 280°C · Resistant to acids, alkalis, and organic solvents
Integrated Conical Bottom + Graduations: Centrifugation and volume reading in a single vessel — eliminates transfer steps
Precise Volume Graduations: Clear, permanent white enamel markings · Key volume points verified for accuracy
High Mechanical Strength: Uniform wall thickness for reliable performance under standard centrifugation (RCF up to 5,000 × g)
Superior Clarity: High-purity glass enables clear visualization of pellet formation
Autoclavable: Steam sterilization at 121°C / 15 psi for 20 minutes
Graduation Range: Covers 5ml to 50ml, meeting diverse laboratory requirements

Value Proposition: One tube = Centrifugation + Quantification. Eliminates post-centrifugation transfer to separate measuring devices, reducing sample loss and handling errors — ideal for precious samples and micro-analysis workflows.

Quality Assurance

Each tube undergoes rigorous dimensional inspection, graduation accuracy verification, and mechanical strength testing. The white enamel markings are permanently fused to the glass surface, ensuring they remain legible through repeated washing and autoclaving cycles. Our products meet laboratory consumable standards, delivering reliable performance for your quantitative centrifugation applications.

Note: Graduations are intended for rapid estimation and volume change monitoring. For precise volumetric measurements, use calibrated volumetric flasks or pipettes. Always inspect tubes for cracks or chips before use, and use appropriate adapters or cushions during centrifugation.

Applications

Typical Application Areas

Biochemistry & Molecular Biology

Direct supernatant volume reading after cell lysis centrifugation · Protein pellet estimation · Nucleic acid elution volume confirmation

Clinical Diagnostics

Serum/plasma volume reading post-blood centrifugation · Urine sediment volume assessment

Microbiology

Bacterial pellet wet weight estimation · Supernatant-to-pellet ratio determination

Pharmaceutical QC

Sedimentation volume ratio testing (pharmacopoeia methods) · Formulation stability studies

Environmental & Food Testing

Quantification of sediment from water samples · Centrifugal quantification in food sample preparation

Compliance & Standards

EP 2.9.35 — Sedimentation Volume Ratio (compatible methodology)
ChP 0982 — Sedimentation Volume Ratio Determination (Chinese Pharmacopoeia)
Compatible with laboratory SOPs requiring combined centrifugation and volume measurement

Tip: Conical graduated tubes are the upgraded version of standard conical tubes, ideal for applications requiring volume change monitoring before and after centrifugation. For best results, pair with RichTech pipettes and volumetric flasks for a complete sample preparation system.

Conical Graduated Centrifuge Tube Operation Guide

03

Preparation Before Use

Inspect each tube for cracks, chips, or rim damage — damaged tubes must never be used for centrifugation
Verify that graduations are clear and legible — no fading or damage
Rinse 2–3 times with distilled water and dry before use (or sterilize as required)
Autoclave conditions: 121°C / 15 psi / 20 minutes

04

Centrifugation Procedure

Counterbalance precisely: Symmetrical placement with weight deviation ≤ 0.1g
Fill volume: Do not exceed 80% of nominal capacity
Rotor matching: Use appropriate rubber cushions or adapters when necessary
Centrifuge settings: Recommended RCF for glass tubes: ≤ 5,000 × g
Acceleration/Braking: Use medium acceleration/deceleration settings

05

Post-Centrifugation Reading

Allow tubes to stand for 2–3 minutes before opening
Read volume at the bottom of the meniscus (standard graduated cylinder reading technique)
Record pre- and post-centrifugation volumes to calculate sediment percentage
For pellet collection, carefully aspirate supernatant without disturbing the pellet

06

Cleaning & Maintenance

Clean immediately after use to prevent residue adhesion
Routine cleaning: Mild detergent + soft brush — rinse 3 times with distilled water
Stubborn residues: Soak in dilute nitric acid or specialized cleaning solution for 2–4 hours
Avoid strong alkaline cleaners or abrasive brushes that may damage graduations
Invert to air dry or place in oven at 60°C until dry
Store upright in dedicated tube racks

Care & Notes

Show care & notes

Safety Precautions

Always secure caps or seal with Parafilm during centrifugation
When working with biohazardous, corrosive, or highly toxic samples, use a biosafety cabinet or fume hood
Glass centrifuge tubes are not suitable for dry-heat sterilization (above 160°C) — use autoclaving

Do not

Never use cracked or damaged centrifuge tubes
Take volume readings under consistent lighting conditions to avoid parallax error