Specifications
Specifications
Component List
All components listed below are included in this kit. Items marked Optional can be customized.
Request custom specs
| Component |
Standard Spec |
Upgrade Options |
Qty |
Link |
| Please contact us to confirm the available specifications. |
📌 Customization Notes:
• Receiver capacity: 1000ml (standard), 2000ml — choose based on volume
• Membranes: MCE (aqueous), Nylon (organic), PTFE (aggressive), RC (general)
• Vacuum pump: Standard or Corrosion-resistant — corrosion-resistant recommended for organic solvents
• Mix and match — no minimum order quantity
• Special requirements? Just ask our technical team
💡 Recommended Add-Ons:
Spare membranes (multiple grades) · GL45 serum bottles (direct receiver/storage) · Vacuum pump spare parts · Solvent bottle adapters
Product Description
I. Why Filter Mobile Phases
HPLC column packing is fine (typically 3–5μm), and the column frit is even finer — 0.5–2μm. Any particles in the mobile phase — undissolved salts, dust, microbes, precipitated buffer salts — will clog that frit. The result? Rising backpressure, poor peak shape, drifting baseline, and in the worst case, a dead column.
Filtering mobile phases costs very little, but it saves a lot — a good HPLC column costs hundreds or thousands, while a filtration setup lasts for years. A quick filtration every day extends column life significantly.
✦ What happens if you don't filter (real stories):
① Backpressure creeps up until the system alarms and shuts down
② Peaks broaden, tail, or show ghost peaks
③ Pump seals wear out — frequent replacements
④ Baseline noise increases — detection limits suffer
⑤ Worst case — a brand new column dies in a month
II. What's in the Setup
- Glass Funnel — 300ml with graduations, for pouring mobile phase
- Fritted Glass Filter Base — 47mm standard size, holds the membrane
- Receiver Flask — 1000ml or 2000ml, collects filtered mobile phase
- Aluminum Clamp — seals funnel to base, prevents leaks
- Silicone Stopper — seals the receiver flask mouth
- Vacuum Pump — oil-free diaphragm type, provides negative pressure
- Vacuum Tubing — connects the base to the pump
III. Choosing the Right Membrane
Two things matter: pore size and material.
- Pore size: 0.45μm is the standard — removes most particles and microbes. 0.22μm is finer — removes bacteria, suitable for biological samples and protein analysis.
- Material:
- MCE (Mixed Cellulose Ester) — aqueous only, inexpensive, hydrophilic
- Nylon — solvent-resistant, good for methanol and acetonitrile
- PTFE — resistant to acids, bases, and all organic solvents — pre-wet required for aqueous
- RC (Regenerated Cellulose) — general-purpose, works with both aqueous and organic
If you're unsure, 0.45μm Nylon is the most versatile — works with acetonitrile, methanol, and water.
IV. Material & Workmanship
The funnel, base, and receiver are all made from borosilicate glass 3.3 — chemically resistant, heat-tolerant, and transparent. The sintered glass frit in the base has uniform pores for flat membrane support. The aluminum clamp is anodized for corrosion resistance and even clamping force.
